In human beings, endometrium undergoes 400–500 menstrual cycles during a woman’s reproductive lifetime. Endometrium is divided into upper two third- functional layer that contains glands and luminal epithelium and the lower basal layer that contains bases of the endometrial glands. Latest theory on endometrial regeneration model postulates that stem cell, possible bone marrow-derived, located in the vascular or perivascular areas, especially around the spiral arterioles in both basal and functional layer contribute to endometrial regeneration. The completion of endometrial regeneration is estrogen dependent, under the stimulation of cytokines and growth factors, such as transforming growth factor-alpha (TGF-α), epidermal growth factor (EGF), platelet-derived growth factor (PDGF), vascular endothelial growth factor (VEGF), insulin-like growth factor-1 (IGF-1), and basic fibroblast growth factor (bFGF). The balance between MMPs and tissue inhibitors of metalloproteinases (TIMPs) play a key role in endometrial remodeling.

Thin endometrial is one cause of menstrual abnormalities, female infertility, implantation failure, recurrent pregnancy loss, and other complications. Adequate endometrial thickness predicts higher implantation rates and lower miscarriage events. Recent therapies to regenerate the endometrium using Platelet-Rich-Plasma (PRP), Growth factors, Stem cell therapies, mesenchymal stromal cell derived- extracellular vesicles have gained importance. All these therapies act through a nearly similar mechanism by local delivery of growth factors, cytokines, miRNAs and their stimulatory effects through paracrine signaling initiating angiogenesis, endothelial regeneration and endothelial remodeling.

HealGen contains the required growth factors like VEGF, PDGF, IGF-1, HGF; miRNAs and ECM proteins (Collagen precursors, MMPs) that help in stimulation of endometrial regeneration and its remodeling. HealGen is an advanced cell free alternative to the existing stem cell therapies which is affordable and a clinical grade solution that helps in restoring fertility.