Wound healing is a complex process and involves spatial and temporal synchronization of a number of cell types that release various growth factors, chemokines, Interleukins with distinct roles in the phases of hemostasis, inflammation, growth, re-epithelialization, and remodeling. Chronic wounds are those that failed to progress through a timely sequence of repair, because of various reasons like infection, impaired angiogenesis, uncontrolled diabetes, unregulated extracellular matrix breakdown by bacterial enzymes etc.

Currently Stem cells therapies for chronic wound healing especially diabetic wounds have attracted substantial clinical significance. The possible therapeutic effects of stem cells are mediated through the release of various growth factors, cytokines and miRNAs which act through the paracrine signaling. Increased angiogenesis has been proposed as one of the main mechanisms of action for stem cell or stem cell conditioned medium in different types of wounds. Pro-angiogenic proteins secreted by MSCs, such as Ang-1, Ang-2, VEGF, angiostatin, CXCL16, EGF, FGF, PDGF, granulocyte-macrophage colony-stimulating factor (GM-CSF), HGF, MCP-1, MMP-8 and MMP-9, contribute to neovascularization and vascular stability. The components like collagen, fibronectin, proteoglycans, hyaluronan, tenascin-C etc, are essential for ECM formation. Collagen precursors and MMPs in the stem cell conditioned medium accelerate the wound healing process by increasing fibroblast migration and proliferation. The components of specially cultured Mesenchymal stromal cell conditioned medium allow proper deposition and orientation of the collagen which is essential for scar free healing.

HealGen is cell free product, derived from fetal tissue contains optimum levels of growth factors like VEGF, PDGF, HGF, IGF-1 which by their paracrine effects, accelerate the wound healing through optimum angiogenesis. HealGen also contains various ECM proteins like collagen precursors and MMPs which help in criss-cross arrangement of collagen leading to reduced scarring and improved tensile strength of the wound. Local infiltration of this product has given promising results in our preclinical animal safety studies. HealGen is an advanced cost effective, cell free, Xeno-free solution that we offer for a No-Option case of chronic non healing wound of critical limb ischemia or diabetic foot ulcer.